Coding
dszA

Part:BBa_K902050:Design

Designed by: Lisa Oberding, Ali Honarmand   Group: iGEM12_Calgary   (2012-10-01)

DBT-sulfone monooxygenase (DszA) from R. erythropolis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 754
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 43
    Illegal NgoMIV site found at 258
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 682
    Illegal AgeI site found at 217
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1066


Design Notes

4 internal PstI cut-sites had to be mutated with site-directed mutagenesis.


Source

Obtained from PCR amplification from a plasmid found in an environmental isolate of R. erythropolis shown to be an active desulfurizer.

References

Gou,Z., Li,X., Xing,J., Liu,H. and Chen,J. LSSE8-1 and its desulfurizational genes. Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 1# Beiertiao, Zhongguancun, Haidian, Beijing 100080, China. 2003.